PCR AMPLIFICATION OF MICRODISSECTED WHEAT CHROMOSOME ARMS IN A SIMPLE SINGLE TUBE REACTION

被引：41

作者：

ALBANI, D ^{[1
]}

COTE, MJ ^{[1
]}

ARMSTRONG, KC ^{[1
]}

CHEN, QF ^{[1
]}

SEGAL, A ^{[1
]}

ROBERT, LS ^{[1
]}

机构：

[1] AGR CANADA,RES BRANCH,PLANT RES CTR,KW NEATBY BLDG,CENT EXPTL FARM,OTTAWA K1A 0C6,ONTARIO,CANADA

来源：

PLANT JOURNAL | 1993年 / 4卷 / 05期

关键词：

D O I：

10.1046/j.1365-313X.1993.04050899.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A novel method for the adaptor-mediated PCR amplification of microdissected chromosome arms is described. This simple and versatile protocol eliminates the need for enzymatic micromanipulation in nanoliter volumes and permits the efficient amplification of as little as two wheat chromosome arms in a 'single tube' reaction.

引用

页码：899 / 903

页数：5

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