CAPILLARY ELECTROPHORESIS OF PROTEINS UNDER ALKALINE CONDITIONS

被引：96

作者：

ZHU, MD ^{[1
]}

RODRIGUEZ, R ^{[1
]}

HANSEN, D ^{[1
]}

WEHR, T ^{[1
]}

机构：

[1] BIORAD LABS,3300 REGATTA BLVD,RICHMOND,CA 94804

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 516卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)90210-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Successful separations of proteins by capillary electrophoresis in uncoated fused-silica capillaries is limited by adsorption and variable rates of electroendosmosis, which can compromise quantitative accuracy and precision. Operation at extremes of pH to minimize these problems is useful in special cases but is not a general strategy for protein separations. Three alternative strategies are described: use of capillaries coated with a linear hydrophilic polymer, the use of acidic solutions to wash the capillary between runs, and the incorporation of additives into the electrophoresis buffer to minimize adsorption during analysis. Applications of these techniques to protein samples is demonstrated. © 1990.

引用

页码：123 / 131

页数：9

共 8 条

[1] CAPILLARY ELECTROPHORESIS OF PROTEINS IN BUFFERS CONTAINING HIGH-CONCENTRATIONS OF ZWITTERIONIC SALTS [J].