AMPEROMETRIC DETERMINATION OF PHOSPHATIDYL CHOLINE IN SERUM WITH USE OF IMMOBILIZED PHOSPHOLIPASE D AND CHOLINE OXIDASE

被引：39
作者：
KARUBE, I
HARA, K
SATOH, I
SUZUKI, S
机构：
[1] Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Midori-ku, Yokohama, 227
来源：
ANALYTICA CHIMICA ACTA | 1979年 / 106卷 / 02期
关键词：
D O I：
10.1016/S0003-2670(01)85007-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Phospholipase D (EC 3.1.4.4.) and choline oxidase (EC 1.1.99.1.) are immobilized together on a hydrophobic agarose gel and used to convert the phospholipid to betaine and hydrogen peroxide, which is measured amperometrically at + 0.60 V vs. SCE. The response time of the sensor is 2 min, and the calibration curve for 0-3 g l-1 of phosphatidyl choline is linear. Different methods of insolubilizing the enzymes are compared. © 1979.
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页码：243 / 250
页数：8
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