FROZEN STORED MURINE HYBRIDOMA CELLS CAN BE USED TO DETERMINE THE VIRULENCE OF LISTERIA-MONOCYTOGENES

被引：13

作者：

BHUNIA, AK

WESTBROOK, DG

STORY, R

JOHNSON, MG

机构：

[1] UNIV ARKANSAS, DEPT FOOD SCI, FOOD SAFETY & HYBRIDOMA LAB, FAYETTEVILLE, AR 72704 USA

[2] UNIV ARKANSAS, CTR BIOTECHNOL, FAYETTEVILLE, AR 72704 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 12期

关键词：

D O I：

10.1128/JCM.33.12.3349-3351.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Murine hybridoma cells, designated Ped-2E9, when stored up to 60 days at -196 degrees C or up to 48 days at -80 degrees C, gave results equivalent to those for freshly grown murine hybridoma cells in an in vitro pathogenicity assay of Listeria species. Thus, laboratories do not need to have their own tissue culture facilities to maintain the hybridoma cells for the assay described.

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页码：3349 / 3351

页数：3

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