CLONING AND EXPRESSION OF A NOVEL FORM OF LEUKOTRIENE B-4 OMEGA-HYDROXYLASE FROM HUMAN LIVER

被引：75
作者：
KIKUTA, Y
KUSUNOSE, E
KONDO, T
YAMAMOTO, S
KINOSHITA, H
KUSUNOSE, M
机构：
[1] FUKUYAMA UNIV,FAC ENGN,DEPT FOOD SCI & TECHNOL,FUKUYAMA,HIROSHIMA 72902,JAPAN
[2] OSAKA CITY UNIV,SCH MED,TONEYAMA INST TB RES,TOYONAKA,OSAKA 560,JAPAN
[3] OSAKA CITY UNIV,SCH MED,DEPT SURG 2,ABENO KU,OSAKA 545,JAPAN
来源：
FEBS LETTERS | 1994年 / 348卷 / 01期
关键词：
CYTOCHROME P-450; LEUKOTRIENE B-4; OMEGA-HYDROXYLASE; CDNA CLONING; HUMAN LIVER;
D O I：
10.1016/0014-5793(94)00587-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated and sequenced a cDNA for human liver LTB(4) omega-hydroxylase. The cDNA encoded a protein of 520 amino acids with a molecular weight of 59,853 Da. The cDNA-deduced amino acid sequence showed 87.3% homology to that of human polymorphonuclear leukocytes (PMN) LTB(4) omega-hydroxylase (CYP4F3). Northern blot analysis revealed that the mRNA hybridized to the specific cDNA fragment is expressed in human liver, but not in human PMN. The microsomes from yeast cells transfected with the cDNA catalyzed the omega-hydroxylation of LTB(4) with a K-m of 44.8 mu M. These results clearly show that a new form of the CYP4F LTB(4) omega-hydroxylase exists in human liver.
引用
收藏
页码：70 / 74
页数：5
相关论文
共 29 条
[1]   ETHANOL-INDUCED INHIBITION OF LEUKOTRIENE DEGRADATION BY OMEGA-OXIDATION [J].
BAUMERT, T ;
HUBER, M ;
MAYER, D ;
KEPPLER, D .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (02) :223-229
[2]  
BRADFIELD JY, 1991, P NATL ACAD SCI USA, V88, P807
[3]   IDENTIFICATION OF A NEW P450 SUBFAMILY, CYP4F1, EXPRESSED IN RAT HEPATIC-TUMORS [J].
CHEN, LP ;
HARDWICK, JP .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (01) :18-23
[4]   STRUCTURAL CHARACTERISTICS OF CYTOCHROME-P-450 - POSSIBLE LOCATION OF THE HEME-BINDING CYSTEINE IN DETERMINED AMINO-ACID-SEQUENCES [J].
GOTOH, O ;
TAGASHIRA, Y ;
IIZUKA, T ;
FUJIIKURIYAMA, Y .
JOURNAL OF BIOCHEMISTRY, 1983, 93 (03) :807-817
[5]   IDENTIFICATION AND BIOLOGICAL-ACTIVITY OF NOVEL OMEGA-OXIDIZED METABOLITES OF LEUKOTRIENE B4 FROM HUMAN-LEUKOCYTES [J].
HANSSON, G ;
LINDGREN, JA ;
DAHLEN, SE ;
HEDQVIST, P ;
SAMUELSSON, B .
FEBS LETTERS, 1981, 130 (01) :107-112
[6]  
HARDWICK JP, 1987, J BIOL CHEM, V262, P801
[7]   HALOTHANE METABOLISM - IMPAIRMENT OF HEPATIC OMEGA-OXIDATION OF LEUKOTRIENES INVIVO AND INVITRO [J].
HUWYLER, J ;
JEDLITSCHKY, G ;
KEPPLER, D ;
GUT, J .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 206 (03) :869-879
[8]   HUMAN GLOMERULAR MESANGIAL CELLS INACTIVATE LEUKOTRIENE-B4 BY REDUCTION INTO DIHYDRO-LEUKOTRIENE-B4 METABOLITES [J].
KAEVER, V ;
BRUUNS, J ;
WUNDER, J ;
DAMERAU, B ;
ZIMMER, G ;
FAULER, J ;
WESSEL, K ;
FLOEGE, J ;
TOPLEY, N ;
RADEKE, H ;
RESCH, K .
LIFE SCIENCES, 1990, 46 (20) :1465-1470
[9]  
KIKUTA Y, 1993, J BIOL CHEM, V268, P9376
[10]   IDENTIFICATION AND BIOLOGICAL-ACTIVITY OF DIHYDRO-LEUKOTRIENE-B4 - A PROMINENT METABOLITE OF LEUKOTRIENE-B4 IN THE HUMAN LUNG [J].
KUMLIN, M ;
FALCK, JR ;
RAUD, J ;
HARADA, Y ;
DAHLEN, SE ;
GRANSTROM, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (01) :23-29
123