SUBSTRUCTURE OF FREEZE-SUBSTITUTED PLASMODESMATA

被引：187

作者：

DING, B ^{[1
]}

TURGEON, R ^{[1
]}

PARTHASARATHY, MV ^{[1
]}

机构：

[1] CORNELL UNIV,DIV BIOL SCI,PLANT BIOL SECT,ITHACA,NY 14853

来源：

PROTOPLASMA | 1992年 / 169卷 / 1-2期

关键词：

FREEZE-SUBSTITUTION; HIGH-PRESSURE FREEZING; INTERCELLULAR TRANSPORT; NICOTIANA-TABACUM; PLASMODESMATA; PROPANE-JET FREEZING;

D O I：

10.1007/BF01343367

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The substructure of plasmodesmata in freeze-substituted tissues of developing leaves of the tobacco plant (Nicotiana tabacum L. var. Maryland Mammoth) was studied by high resolution electron microscopy and computer image enhancement techniques. Both the desmotubule wall and the inner leaflet of the plasmodesmatal plasma membrane are composed of regularly spaced electron-dense particles approximately 3 nm in diameter, presumably proteinaceous and embedded in lipid. The central rod of the desmotubule is also particulate. In plasmodesmata with central cavities, spoke-like extensions are present between the desmotubule and the plasma membrane in the central cavity region. The space between the desmotubule and the plasma membrane appears to be the major pathway for intercellular transport through plasmodesmata. This pathway may be tortuous and its dimensions could be regulated by interactions between desmotubule and plasma membrane particles.

引用

页码：28 / 41

页数：14

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