THE USE OF RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS IN WHEAT

被引：323

作者：

DEVOS, KM

GALE, MD

机构：

[1] Cambridge Laboratory, Norwich, NR4 7UJ, Colney Lane

来源：

THEORETICAL AND APPLIED GENETICS | 1992年 / 84卷 / 5-6期

关键词：

RAPD; PCR; MARKERS; WHEAT; TRITICUM-AESTIVUM;

D O I：

10.1007/BF00224153

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An evaluation was made of the use of random amplified polymorphic DNA (RAPD) as a genetic marker system in wheat. Reproducible amplification products were obtained from varietal, homozygous single chromosome recombinant line and wheat/alien addition line genomic DNA with selected primers and rigorously optimized reaction conditions. Factors influencing the RAPD patterns are DNA concentration, Mg2+ concentration, polymerase concentration and denaturing temperature. In wheat, the non-homoeologous, non-dose responsive and dominant behaviour of RAPD products devalues their use as genetic markers for the construction of linkage maps, and the high probability that the amplified fragments derive from repetitive DNA limits their use as a source of conventional RFLP probes. However, RAPD markers will most certainly find many applications in the analysis of genotypes where single chromosomes or chromosome segments are to be manipulated.

引用

页码：567 / 572

页数：6

共 14 条

[11] IDENTIFICATION AND MAPPING OF POLYMORPHISMS IN CEREALS BASED ON THE POLYMERASE CHAIN-REACTION [J].