MAPPING T-CELL RECEPTOR PEPTIDE CONTACTS BY VARIANT PEPTIDE IMMUNIZATION OF SINGLE-CHAIN TRANSGENICS

被引：529

作者：

JORGENSEN, JL ^{[1
]}

ESSER, U ^{[1
]}

FAZEKAS DE ST GROTH, B ^{[1
]}

REAY, PA ^{[1
]}

DAVIS, MM ^{[1
]}

机构：

[1] STANFORD UNIV, HOWARD HUGHES MED INST, STANFORD, CA 94305 USA

来源：

NATURE | 1992年 / 355卷 / 6357期

关键词：

D O I：

10.1038/355224a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To test models of T-cell recognition, mice transgenic for T-cell receptor-alpha or beta-chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V(alpha) or V(beta) chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V(beta) response into a very heterogeneous one.

引用

页码：224 / 230

页数：7

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