H-1-NMR STUDY ON UNFOLDING OF TRICHOSANTHIN

被引：0

作者：

HU, HY ^{[1
]}

LU, ZX ^{[1
]}

DU, YC ^{[1
]}

机构：

[1] ACAD SINICA,SHANGHAI INST BIOCHEM,SHANGHAI 200031,PEOPLES R CHINA

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 1995年 / 27卷 / 02期

关键词：

TRICHOSANTHIN; HIS(51) RESIDUE; H-1; NMR; UNFOLDING;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The unfolding of Trichosanthin (TCS) under acidic pH and guanidine chloride (GuHCl) has been investigated by H-1 NMR methods. The variation of C2H chemical shift by pH and GuHCl titration indicates that His(51) locates in the hydrophobic core of the large domain of TCS and an intermediate state is proposed during the acidic unfolding process. In alkaline condition (pH 12.0), It is also observed by 2D NOESY that the large domain still contains hydrophobic region while the small domain is random.

引用

页码：229 / 232

页数：4

共 6 条

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