EVALUATION OF A NEW CYTOTOXICITY ASSAY FOR CLOSTRIDIUM-PERFRINGENS TYPE-D EPSILON-TOXIN

被引：1

作者：

PAYNE, DW

WILLIAMSON, ED

HAVARD, H

MODI, N

BROWN, J

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1994年 / 116卷 / 02期

关键词：

CLOSTRIDIUM PERFRINGENS; CYTOTOXICITY ASSAY; EPSILON TOXIN; MTS; MADIN DARBY CANINE KIDNEY;

D O I：

10.1111/j.1574-6968.1994.tb06695.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new cytotoxicity assay for determining the activity of epsilon toxin produced by Clostridium perfringens type D has been developed. Viability of cultured cells was determined by the ability of only live cells to convert 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulfophenyl)tetrazolium to the coloured product formazan in the presence of phenazine methosulfate. Of the 12 cell lines tested, only the MDCK cell line was susceptible to epsilon toxin. Specificity was confirmed by the ability of only specific monoclonal antibodies to inhibit cytotoxicity. Good correlation was obtained with the mouse lethality assay (r = 0.991) and over a wide range of viability (15-75%) as determined by ethidium bromide/acridine orange staining (r = 0.995).

引用

页码：161 / 167

页数：7

共 21 条

[1] 5-(3-CARBOXYMETHOXYPHENYL)-2-(4,5-DIMETHYLTHIAZOLYL)-3-(4-SULFOPHENYL)TETRAZOLIUM, INNER SALT (MTS) AND RELATED ANALOGS OF 3-(4,5-DIMETHYLTHIAZOLYL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) REDUCING TO PURPLE WATER-SOLUBLE FORMAZANS AS CELL-VIABILITY INDICATORS [J].