AUTOMATED IMAGE-ANALYSIS DNA CYTOMETRY IN TESTICULAR CANCER

被引：0

作者：

BURGER, RA ^{[1
]}

WITZSCH, U ^{[1
]}

ENGEL, H ^{[1
]}

POTRATZ, D ^{[1
]}

STOCKLE, M ^{[1
]}

机构：

[1] JOHANNES GUTENBERG UNIV,PATHOL KLIN,D-55131 MAINZ,GERMANY

来源：

UROLOGICAL RESEARCH | 1994年 / 22卷 / 01期

关键词：

TESTICULAR TUMOR; AUTOMATED IMAGE ANALYSIS; DNA CYTOMETRY; CLASSIFICATION; SEMINOMA; EMBRYONAL CARCINOMA;

D O I：

暂无

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

The value of automated DNA cytometry for differentiation of testis cancer was evaluated in 54 seminomas, 13 HCG-positive seminomas, and 48 embryonal carcinomas. Slices of paraffin embedded tissue were enzymatically digested and stained with Feulgen SITS after fixation on glass slides. Automated DNA cytometry was performed with a Modular Image Analysis Computer (MIAC). DNA histogram phenotpye and computed DNA indices were correlated with the different tumor types. The ratio of hypertriploid to hypotriploid increased from HCG-positive seminoma over embryonal carcinoma to seminoma. The following mathematical DNA indices were found to correlate with tumor type: mean ploidy, 2c deviation index, 5c exceeding rate, variation coefficient of the GO/1 fraction and DNA nucleus diameter correlation.

引用

页码：17 / 20

页数：4

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