ATP-INHIBITABLE CL- CHANNEL IN APICAL MEMBRANES OF CULTURED RABBIT CORTICAL COLLECTING DUCT CELLS

被引:11
作者
SUPERDOCK, KR
SNYDERS, DJ
BREYER, MD
机构
[1] VET AFFAIRS MED CTR,F427 ACRE BLDG,NASHVILLE,TN 37212
[2] VANDERBILT UNIV,DEPT MED,DIV NEPHROL,NASHVILLE,TN 37212
[3] VANDERBILT UNIV,DEPT CLIN PHARMACOL,NASHVILLE,TN 37212
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 265卷 / 04期
关键词
PATCH CLAMP; EPITHELIA;
D O I
10.1152/ajpcell.1993.265.4.C957
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Excised patches of apical membranes from immunodissected rabbit cortical collecting duct cells in primary culture were studied by the patch-clamp technique. Barium (1 mM) and tetraethylammonium chloride (5 mM) were added to all solutions to block potassium channel activity. A unique channel was observed that exhibited inward rectification under symmetrical ionic conditions with a measured chord conductance of 54.0 +/- 2.5 pS at - 80 mV (n = 11) and 22.1 +/- 1.7 pS at +80 mV (n = 5). This channel was chloride selective, with a P(Na):P(Cl) of 0.16 (n = 3). Kinetic analysis revealed a voltage-independent open-time probability of 0.80 +/- 0.07 (n = 6). Open-time probability within bursts was 0.96 +/- 0.01. Addition of ATP to the cytosolic surface of the channel resulted in a dose-dependent decrease in open probability, with a threshold effect at 10(-4) M. due to a reduction in burst open time. The effect of ATP was immediate, rapidly reversible at room temperature, and mimicked by GTP, adenosine 5'-O-(3-thiotriphosphate), and guanosine 5-O-(3-thiotriphosphate). This channel may link epithelial chloride permeability to cellular ATP content in the rabbit cortical collecting duct.
引用
收藏
页码:C957 / C965
页数:9
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