DIETHYLDITHIOCARBAMATE INHIBITS INDUCTION OF MACROPHAGE NO SYNTHASE

被引：139

作者：

MULSCH, A ^{[1
]}

SCHRAYUTZ, B ^{[1
]}

MORDVINTCEV, PI ^{[1
]}

HAUSCHILDT, S ^{[1
]}

BUSSE, R ^{[1
]}

机构：

[1] UNIV FREIBURG,DEPT IMMUNOBIOL,W-7800 FREIBURG,GERMANY

来源：

FEBS LETTERS | 1993年 / 321卷 / 2-3期

关键词：

NO SYNTHASE; TRANSCRIPTION; NFKAPPAB; DIETHYLDITHIOCARBAMATE; BONE MARROW-DERIVED MACROPHAGE;

D O I：

10.1016/0014-5793(93)80111-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated whether sodium diethyldithiocarbamate (DETC), an inhibitor of the nuclear transcription factor kappa B (NFkappaB), modulates induction of NO synthase (NOS) in murine bone marrow-derived macrophages. A short exposure (between 1 and 16 h) of L929-cell medium-preconditioned macrophages to E. coli lipopolysaccharide (LPS) significantly increased the level of NOS mRNA, and elicited NO formation as detected by electron spin resonance spectroscopy and by the release of nitrite. DETC (0.1-1 mM) present during stimulation with LPS prevented the increase in NOS mRNA and the expression of NOS activity. These findings suggest that NFkappaB is involved in the signal transduction pathway linking stimulation of macrophages by LPS with transcription of the gene encoding inducible NOS.

引用

页码：215 / 218

页数：4

共 15 条

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