A COMPARISON BETWEEN LOW BACKGROUND SILVER DIAMMINE AND SILVER-NITRATE PROTEIN STAINS

被引：223

作者：

RABILLOUD, T ^{[1
]}

机构：

[1] INST PASTEUR,DEPT IMMUNOL,F-75724 PARIS 15,FRANCE

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 07期

关键词：

D O I：

10.1002/elps.1150130190

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Several methods of silver staining of proteins after sodium dodecyl sulfate-electrophoresis in polyacrylamide gels were compared. The most rapid methods were found to be less sensitive than more time-consuming methods. Among the long methods, those using glutaraldehyde treatment of the gel and silver diammine complex as the silvering agent were found to be the most sensitive, at the expense of the use of a modified polyacrylamide matrix and higher silver concentrations.

引用

页码：429 / 439

页数：11

共 39 条

[21] SILVER STAINING OF HISTONES IN TRITON ACID UREA GELS [J].

MOLD, DE ;

WEINGART, J ;

ASSARAF, J ;

LUBAHN, DB ;

KELNER, DN ;

SHAW, BR ;

MCCARTY, KS .

ANALYTICAL BIOCHEMISTRY, 1983, 135 (01) :44-47

[22] SILVER STAIN FOR PROTEINS IN POLYACRYLAMIDE GELS - A MODIFIED PROCEDURE WITH ENHANCED UNIFORM SENSITIVITY [J].

MORRISSEY, JH .

ANALYTICAL BIOCHEMISTRY, 1981, 117 (02) :307-310

[23] A SIMPLIFIED ULTRASENSITIVE SILVER STAIN FOR DETECTING PROTEINS IN POLYACRYLAMIDE GELS [J].

OAKLEY, BR ;

KIRSCH, DR ;

MORRIS, NR .

ANALYTICAL BIOCHEMISTRY, 1980, 105 (02) :361-363

[24] SILVER STAINS FOR PROTEINS IN POLYACRYLAMIDE GELS - A COMPARISON OF 6 METHODS [J].

OCHS, DC ;

MCCONKEY, EH ;

SAMMONS, DW .

ELECTROPHORESIS, 1981, 2 (5-6) :304-307

[25]

OFARRELL PH, 1975, J BIOL CHEM, V250, P4007

[26] HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF BASIC AS WELL AS ACIDIC PROTEINS [J].

OFARRELL, PZ ;

GOODMAN, HM ;

OFARRELL, PH .

CELL, 1977, 12 (04) :1133-1141

[27] IMPROVEMENT AND SIMPLIFICATION OF LOW-BACKGROUND SILVER STAINING OF PROTEINS BY USING SODIUM DITHIONITE [J].

RABILLOUD, T ;

CARPENTIER, G ;

TARROUX, P .

ELECTROPHORESIS, 1988, 9 (06) :288-291

[28] MECHANISMS OF PROTEIN SILVER STAINING IN POLYACRYLAMIDE GELS - A 10-YEAR SYNTHESIS [J].

RABILLOUD, T .

ELECTROPHORESIS, 1990, 11 (10) :785-794

[29] QUANTITATION OF MICROGRAM AMOUNTS OF PROTEIN IN TWO-DIMENSIONAL POLYACRYLAMIDE-GEL ELECTROPHORESIS SAMPLE BUFFER [J].

RAMAGLI, LS ;

RODRIGUEZ, LV .

ELECTROPHORESIS, 1985, 6 (11) :559-563

[30] ULTRASENSITIVE SILVER-BASED COLOR STAINING OF POLYPEPTIDES IN POLYACRYLAMIDE GELS [J].

SAMMONS, DW ;

ADAMS, LD ;

NISHIZAWA, EE .

ELECTROPHORESIS, 1981, 2 (03) :135-141

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