BENZOYLATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETECTION OF SOLASODINE

被引：8
作者：
DREWES, FE
VANSTADEN, J
DREWES, SE
机构：
[1] UNIV NATAL, UN FRD PLANT GROWTH & DEV RES UNIT, PIETERMARITZBURG 3201, SOUTH AFRICA
[2] UNIV NATAL, DEPT CHEM, PIETERMARITZBURG 3201, SOUTH AFRICA
来源：
PHYTOCHEMICAL ANALYSIS | 1992年 / 3卷 / 02期
关键词：
BENZOYLATION; DERIVATIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SOLANUM; SOLASODINE; STEROIDAL ALKALOIDS;
D O I：
10.1002/pca.2800030209
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid technique for the determination of solasodine by high performance liquid chromatography (HPLC) following derivatization of the steroidal alkaloid by benzoyl chloride has been developed. In the presence of a 5 M excess of benzoyl chloride, the reaction proceeds to completion in 6 h at 50-degrees-C. The levels of benzoylated solasodine can be determined by HPLC at a wavelength of 254 nm, there being a linear relationship between the amount of solasodine injected and the peak area produced. Microgram quantities of solasodine can be detected in this way. The benzoylated product is very stable.
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页码：85 / 87
页数：3
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