TRANSFORMING GROWTH-FACTOR-BETA REGULATES THE SPLICING PATTERN OF FIBRONECTIN MESSENGER-RNA PRECURSOR

被引：120

作者：

BORSI, L ^{[1
]}

CASTELLANI, P ^{[1
]}

RISSO, AM ^{[1
]}

LEPRINI, A ^{[1
]}

ZARDI, L ^{[1
]}

机构：

[1] IST NAZL RIC CANC,CELL BIOL LAB,VIALE BENEDETTO XV 10,I-16132 GENOA,ITALY

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

Fibronectin isoform; Pre-mRNA splicing regulation; Transforming growth factor-β;

D O I：

10.1016/0014-5793(90)80664-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fibronectin (FN) polymorphism is caused by alternative splicing patterns in at least three regions (ED-A, ED-B and IIICS) of the primary transcript of a single gene. Using monoclonal antibodies, we previously demonstrated that transforming growth factor-β (TGF-β) preferentially increases the accumulation of the FN isoforms containing the ED-A sequence in cultured normal human fibroblasts [Balza et al., (1988) FEBS Lett. 228, 42-44]. To determine the basis of this effect, we have examined through S1 nuclease analysis, the levels of ED-A- and ED-B-containing mRNAs in cultured normal human skin flbroblasts before and after TGF-β treatment. These experiments have shown that TGF-β increases the relative amount of m-RNA for ED-A- and ED-B-containing FN isoforms. These data demonstrate that a growth factor may regulate the splicing pattern of a pre-mRNA. © 1990.

引用

页码：175 / 178

页数：4