PHYSICAL MAP OF THE CHANNEL CATFISH VIRUS GENOME - LOCATION OF SITES FOR RESTRICTION ENDONUCLEASES ECORI, HINDIII, HPAI, AND XBAI

The overall arrangement of nucleotide sequences in the DNA of channel catfish virus was studied by cleavage with 4 restriction endonucleases. Physical maps were developed for the location of sites for EcoRI, HindIII, HpaI and XbaI. The sum of the MW of fragments generated by each restriction enzyme indicates a MW of about 86 .times. 106 for the channel catfish virus genome. Fragments corresponding to the molecular ends of channel catfish virus DNA were identified by their sensitivity to exonuclease treatment. The distribution of restriction sites in the genome showed that sequences included in a 12 .times. 106-MW region at 1 end are repeated with direct polarity at the other end and that the overall genomic sequence order is nonpermuted.