AQUEOUS 2-PHASE SYSTEMS IN BIOCHEMICAL RECOVERY - SYSTEMATIC ANALYSIS, DESIGN, AND IMPLEMENTATION OF PRACTICAL PROCESSES FOR THE RECOVERY OF PROTEINS

被引：54
作者：
HUDDLESTON, JG [1 ]
LYDDIATT, A [1 ]
机构：
[1] UNIV BIRMINGHAM, SCH CHEM ENGN, BIOCHEM RECOVERY GRP, BIRMINGHAM B15 2TT, W MIDLANDS, ENGLAND
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1990年 / 26卷 / 03期
关键词：
Aqueous two-phase systems; dextran; extraction; partition; poly(ethylene glycol); protein recovery; purification; separation;
D O I：
10.1007/BF02921506
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The design and application of aqueous two-phase systems for recovery of proteins is reviewed. Reference is made to the properties of polymers and salt forming the systems and their influence on phase separation and partition. The properties of systems important for the design of purification strategies are discussed in relation to the surface properties of proteins. Strategies for the modification of systems for bioaffinity partition are considered including the choice of carrier in terms of molecular type and properties, or by particulate addition. Finally, the scaleup of partition is considered, and the reasons for currently limited adoption at larger scales are elucidated. © 1990 Humana Press Inc.
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页码：249 / 279
页数：31
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