ISOLATION AND TRANSLATION OF ELASTIN MESSENGER-RNA FROM CHICK AORTA

被引：71

作者：

BURNETT, W ^{[1
]}

ROSENBLOOM, J ^{[1
]}

机构：

[1] UNIV PENN,SCH DENT MED,DEPT HISTOL & EMBRYOL,PHILADELPHIA,PA 19104

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91739-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mRNA was isolated from 15-day chick embryo aortae by digestion of the tissue at 40° with Proteinase K in 1% sodium dodecyl sulfate followed by chromatography on oligo(dT)-cellulose columns. The mRNA was translated using a reticulocyte lysate system and the tropoelastin immunoprecipitated using a specific antiserum prepared against insoluble elastin. The precipitated product was characterized by polyacrylamide gel electrophoresis and a major peak corresponding to a molecular weight of approximately 70,000 daltons was observed. No higher molecular weight product was observed. © 1979.

引用

页码：478 / 484

页数：7

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