A COMPLEX BETWEEN E2F AND THE PRB-RELATED PROTEIN P130 IS SPECIFICALLY TARGETED BY THE SIMIAN-VIRUS-40 LARGE T-ANTIGEN DURING CELL-TRANSFORMATION

被引：0
作者：
WOLF, DA
HERMEKING, H
ALBERT, T
HERZINGER, T
KIND, P
EICK, D
机构：
[1] GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT,INST KLIN MOLEK BIOL & TUMORGENET,D-81377 MUNICH,GERMANY
[2] UNIV MUNICH,DERMATOL KLIN & POLIKLIN,MOLEK PATHOL ABT,D-80337 MUNICH,GERMANY
来源：
ONCOGENE | 1995年 / 10卷 / 11期
关键词：
TRANSFORMATION; E2F; P130; SV40 LARGE T ANTIGEN; CELL CYCLE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The p130 protein is a recently cloned member of the retinoblastoma protein family. We show here that transformation of NIH3T3-L1 fibroblasts (L1 cells) by the simian virus 40 large T antigen (LTAg) depends on the disruption of DNA binding complexes between transcription factor E2F and p130. LTAg binds to the pocket region of p130 in vivo and disrupts the E2F-p130 complexes. E2F-p130 complexes are present only in quiescent L1 cells and disappear at the G1/S phase boundary concomitantly to induction of DNA synthesis and expression of the E2F-regulated cdc2 gene. p130 is a substrate of cyclin-dependent kinase 2 (Cdk2) in vitro and associates with a Cdk in vivo which is activated upon serum stimulation in late G1. Overexpression of p130 inhibits cdc2 promoter activity and entry of quiescent L1 cells into S phase. The results demonstrate that p130 is a negative regulator of cell cycle progression which is specifically targeted by LTAg during cell transformation.
引用
收藏
页码：2067 / 2078
页数：12
相关论文
共 59 条
[1]   THE RETINOBLASTOMA PROTEIN COPURIFIES WITH E2F-I, AN E1A-REGULATED INHIBITOR OF THE TRANSCRIPTION FACTOR E2F [J].
BAGCHI, S ;
WEINMANN, R ;
RAYCHAUDHURI, P .
CELL, 1991, 65 (06) :1063-1072
[2]   FUNCTIONAL SYNERGY BETWEEN DP-1 AND E2F-1 IN THE CELL CYCLE-REGULATING TRANSCRIPTION FACTOR DRTF1/E2F [J].
BANDARA, LR ;
BUCK, VM ;
ZAMANIAN, M ;
JOHNSTON, LH ;
LATHANGUE, NB .
EMBO JOURNAL, 1993, 12 (11) :4317-4324
[3]   E2F-4, A NEW MEMBER OF THE E2F GENE FAMILY, HAS ONCOGENIC ACTIVITY AND ASSOCIATES WITH P107 IN-VIVO [J].
BEIJERSBERGEN, RL ;
KERKHOVEN, RM ;
ZHU, LA ;
CARLEE, L ;
VOORHOEVE, PM ;
BERNARDS, R .
GENES & DEVELOPMENT, 1994, 8 (22) :2680-2690
[4]   THE E2F TRANSCRIPTION FACTOR IS A CELLULAR TARGET FOR THE RB PROTEIN [J].
CHELLAPPAN, SP ;
HIEBERT, S ;
MUDRYJ, M ;
HOROWITZ, JM ;
NEVINS, JR .
CELL, 1991, 65 (06) :1053-1061
[5]   CELL-CYCLE ANALYSIS OF E2F IN PRIMARY HUMAN T-CELLS REVEALS NOVEL E2F COMPLEXES AND BIOCHEMICALLY DISTINCT FORMS OF FREE E2F [J].
CHITTENDEN, T ;
LIVINGSTON, DM ;
DECAPRIO, JA .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (07) :3975-3983
[6]   CELL CYCLE-SPECIFIC ASSOCIATION OF E2F WITH THE P130 E1A-BINDING PROTEIN [J].
COBRINIK, D ;
WHYTE, P ;
PEEPER, DS ;
JACKS, T ;
WEINBERG, RA .
GENES & DEVELOPMENT, 1993, 7 (12A) :2392-2404
[7]   CELL-CYCLE REGULATION OF THE HUMAN CDC2 GENE [J].
DALTON, S .
EMBO JOURNAL, 1992, 11 (05) :1797-1804
[8]   HUMAN PAPILLOMAVIRUS TYPE-16 E7 ASSOCIATES WITH A HISTONE H1 KINASE AND WITH P107 THROUGH SEQUENCES NECESSARY FOR TRANSFORMATION [J].
DAVIES, R ;
HICKS, R ;
CROOK, T ;
MORRIS, J ;
VOUSDEN, K .
JOURNAL OF VIROLOGY, 1993, 67 (05) :2521-2528
[9]   SV40 LARGE TUMOR-ANTIGEN FORMS A SPECIFIC COMPLEX WITH THE PRODUCT OF THE RETINOBLASTOMA SUSCEPTIBILITY GENE [J].
DECAPRIO, JA ;
LUDLOW, JW ;
FIGGE, J ;
SHEW, JY ;
HUANG, CM ;
LEE, WH ;
MARSILIO, E ;
PAUCHA, E ;
LIVINGSTON, DM .
CELL, 1988, 54 (02) :275-283
[10]   A CYCLIN-A-PROTEIN KINASE COMPLEX POSSESSES SEQUENCE-SPECIFIC DNA-BINDING ACTIVITY - P33CDK2 IS A COMPONENT OF THE E2F-CYCLIN-A COMPLEX [J].
DEVOTO, SH ;
MUDRYJ, M ;
PINES, J ;
HUNTER, T ;
NEVINS, JR .
CELL, 1992, 68 (01) :167-176
123456