FLOW CYTOMETRIC EVALUATION OF THE ACROSOME REACTION OF HUMAN SPERMATOZOA - A NEW METHOD USING A PHOTOACTIVATED SUPRAVITAL STAIN

被引：16

作者：

HENLEY, N ^{[1
]}

BARON, C ^{[1
]}

ROBERTS, KD ^{[1
]}

机构：

[1] UNIV MONTREAL, HOP MAISON NEUVE ROSEMONT, RES CTR, MONTREAL H1T 2M4, PQ, CANADA

来源：

INTERNATIONAL JOURNAL OF ANDROLOGY | 1994年 / 17卷 / 02期

关键词：

ACROSOME REACTION; ETHIDIUM MONOAZIDE; FLOW CYTOMETRY; SPERMATOZOA;

D O I：

10.1111/j.1365-2605.1994.tb01224.x

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

A flow cytometric assay using a double-stain method for the measurement of the acrosome reaction of human spermatozoa is described. The use of a stable photoactivated stain, ethidium monoazide, allowed evaluation of the viability of spermatozoa. This stain was more stable in fixed samples than propidium iodide, which is not bound covalently to DNA and is therefore removed readily during the washing procedure. The permeabilized acrosome nas labelled with Pisum sativum agglutinin conjugated with fluoroisothiocyanate. Since this lectin binds to the acrosome and acrosomal contents, a decrease in the fluorescence intensity allows the cytometric evaluation of the acrosome reaction. Microscopic analysis and now cytometric analysis were well correlated and cell sorting was performed to ensure the homogeneity of each different subpopulation encountered.

引用

页码：78 / 84

页数：7

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