NONINVASIVE MEASUREMENT OF GLUCOSE-UPTAKE BY PREIMPLANTATION SHEEP EMBRYOS AND UNFERTILIZED OVA

被引:3
作者
BUTLER, JE
WILLIAMS, JE
机构
[1] Department of Animal Science, University of Idaho, Moscow
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0921-4488(92)90167-3
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Using a noninvasive, fluorometric assay, glucose uptake was measured in individual preimplantation sheep embryos and unfertilized ova. Glucose uptake remained low through the morula stage of development. By the late morula stage, glucose uptake began to increase and was significantly higher by the blastocyst stage (P < 0.08). This pattern was similar to that reported in mice.
引用
收藏
页码:347 / 352
页数:6
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共 17 条
  • [1] Anderson, Bradford, Cupps, Length of gestation in ewes carrying lambs of two different breeds, Theriogenology, 16, pp. 119-129, (1981)
  • [2] Barbehenn, Wales, Lowry, The explanation for the blockade of glycolysis in early mouse embryos, Proc. Natl. Acad. Sci. USA, 71, pp. 1056-1069, (1974)
  • [3] Bondioli, Wright, Superovulation of progestin synchronized ewes, Theriogenology, 13, (1980)
  • [4] Brinster, Incorporation of carbon from glucose and pyruvate into the preimplantation mouse embryo, Expt. Cell. Res., 58, pp. 153-158, (1969)
  • [5] Butler, Lechene, Biggers, Noninvasive measurement of glucose uptake by two populations of murine embryos, Biol. Reprod., 39, pp. 779-786, (1988)
  • [6] Flood, Wiebold, Glucose metabolism by preimplantation pig embryos, J. Reprod. Fert., 84, pp. 7-12, (1988)
  • [7] Gardner, Leese, Non-invasive measurement of nutrient uptake by single cultured pre-implantation mouse embryos, Hum. Reprod., 1, pp. 25-27, (1986)
  • [8] Leese, Barton, Pyruvate and glucose uptake by mouse ova and preimplantation embryos, J. Reprod. Fert., 72, pp. 9-13, (1984)
  • [9] Leese, Biggers, Mroz, Lechene, Nucleotides in a single mammalian ovum or preimplantation embryo, Anal. Biochem., 140, pp. 443-448, (1984)
  • [10] Mroz, Lechene, Fluorescence analysis of picoliter samples, Anal. Biochem., 102, pp. 90-96, (1980)