HEPATOCYTE GROWTH-FACTOR IS A POTENT STIMULATOR OF HUMAN MELANOCYTE DNA-SYNTHESIS AND GROWTH

被引：217
作者：
MATSUMOTO, K
TAJIMA, H
NAKAMURA, T
机构：
[1] Department of Biology, Faculty of Science, Kyushu University, Fukuoka
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期
关键词：
D O I：
10.1016/0006-291X(91)90887-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hepatocyte growth factor (HGF) is a potent mitogen for adult rat hepatocytes in primary culture. HGF stimulates growth and DNA synthesis of normal human epidermal melanocytes in culture. The maximal stimulation of DNA synthesis by 4.0-fold occurred with 10 ng/ml HGF. This stimulatory effect was additive with both acidic and basic fibroblast growth factors, while it was inhibited by transforming growth factor-β1. Melanocytes expressed a single class of specific, high-affinity receptors for HGF with a Kd of 22 pM and approximately 120 receptors/cell. Thus, HGF is a potent mitogen for normal human epidermal melanocytes. © 1991.
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页码：45 / 51
页数：7
相关论文
共 22 条
[11]   MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR HUMAN HEPATOCYTE GROWTH-FACTOR [J].
MIYAZAWA, K ;
TSUBOUCHI, H ;
NAKA, D ;
TAKAHASHI, K ;
OKIGAKI, M ;
ARAKAKI, N ;
NAKAYAMA, H ;
HIRONO, S ;
SAKIYAMA, O ;
TAKAHASHI, K ;
GOHDA, E ;
DAIKUHARA, Y ;
KITAMURA, N .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 163 (02) :967-973
[12]   MOLECULAR-CLONING AND EXPRESSION OF HUMAN HEPATOCYTE GROWTH-FACTOR [J].
NAKAMURA, T ;
NISHIZAWA, T ;
HAGIYA, M ;
SEKI, T ;
SHIMONISHI, M ;
SUGIMURA, A ;
TASHIRO, K ;
SHIMIZU, S .
NATURE, 1989, 342 (6248) :440-443
[13]   PURIFICATION AND CHARACTERIZATION OF A GROWTH-FACTOR FROM RAT PLATELETS FOR MATURE PARENCHYMAL HEPATOCYTES IN PRIMARY CULTURES [J].
NAKAMURA, T ;
TERAMOTO, H ;
ICHIHARA, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) :6489-6493
[14]   2 TYPES OF GROWTH INHIBITOR IN RAT PLATELETS FOR PRIMARY CULTURED RAT HEPATOCYTES [J].
NAKAMURA, T ;
TERAMOTO, H ;
TOMITA, Y ;
ICHIHARA, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 134 (02) :755-763
[15]   PURIFICATION AND SUBUNIT STRUCTURE OF HEPATOCYTE GROWTH-FACTOR FROM RAT PLATELETS [J].
NAKAMURA, T ;
NAWA, K ;
ICHIHARA, A ;
KAISE, N ;
NISHINO, T .
FEBS LETTERS, 1987, 224 (02) :311-316
[16]   PARTIAL-PURIFICATION AND CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM SERUM OF HEPATECTOMIZED RATS [J].
NAKAMURA, T ;
NAWA, K ;
ICHIHARA, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 122 (03) :1450-1459
[17]   EXPRESSION OF HEPATOCYTE GROWTH-FACTOR GENE IN ENDOTHELIAL AND KUPFFER CELLS OF DAMAGED RAT LIVERS, AS REVEALED BY INSITU HYBRIDIZATION [J].
NOJI, S ;
TASHIRO, K ;
KOYAMA, E ;
NOHNO, T ;
OHYAMA, K ;
TANIGUCHI, S ;
NAKAMURA, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 173 (01) :42-47
[18]   SERUM-FREE CULTURE OF NORMAL HUMAN MELANOCYTES - GROWTH-KINETICS AND GROWTH-FACTOR REQUIREMENTS [J].
PITTELKOW, MR ;
SHIPLEY, GD .
JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 140 (03) :565-576
[19]   MITOTIC-ACTIVITY OF EPIDERMAL MELANOCYTES IN UV-IRRADIATED MOUSE SKIN [J].
ROSDAHL, IK ;
SZABO, G .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1978, 70 (03) :143-148
[20]  
SHIPLEY GD, 1986, CANCER RES, V46, P2068
123