ANALYSIS OF LOWER ALIPHATIC-ALDEHYDES IN WATER BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY WITH DERIVATIZATION TO 2,4-DINITROPHENYLHYDRAZONES

被引：26
作者：
TAKEDA, S
WAKIDA, S
YAMANE, M
HIGASHI, K
机构：
[1] Osaka National Research Institute, AIST, Osaka
来源：
ELECTROPHORESIS | 1994年 / 15卷 / 10期
关键词：
D O I：
10.1002/elps.11501501203
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The analysis of lower aliphatic aldehydes in water following derivatization to 2,4-dinitrophenylhydrazones, was investigated by micellar electrokinetic chromatography (MEKC). The effects of the buffer pH and acetonitrile addition on the separation were investigated. Under optimized conditions the 2,4-dinitrophenylhydrazine (DNPH) reagent was completely separated from four DNPH derivatized aldehydes with high separation efficiency. DNPH-formaldehyde and DNPH-acetaldehyde were also completely separated from one another. Their relative standard deviation of migration times and peak areas were within 1.0% and 3.3%, respectively. The reproducibility of peak areas in this MEKC analysis was as good as that of high-performance liquid chromatography (HPLC) analysis with the same derivatization method. The recovery values from tap and river water were in the range of 97-102%. The detection limits of formaldehyde and acetaldehyde were 0.05 mg/L and 0.08 mg/L respectively.
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页码：1332 / 1334
页数：3
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