DIADENOSINE 5',5'''-P-1,P-4-TETRAPHOSPHATE (AP(4)A), ATP AND CATECHOLAMINE CONTENT IN BOVINE ADRENAL-MEDULLA, CHROMAFFIN GRANULES AND CHROMAFFIN CELLS

被引：0

作者：

SILLERO, MAG ^{[1
]}

DELVALLE, M ^{[1
]}

ZAERA, E ^{[1
]}

MICHELENA, P ^{[1
]}

GARCIA, AG ^{[1
]}

SILLERO, A ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC MED,DEPT FARMACOL & TERAPEUT,E-28029 MADRID,SPAIN

来源：

BIOCHIMIE | 1994年 / 76卷 / 05期

关键词：

DIADENOSINE TETRAPHOSPHATE; CATECHOLAMINES; ADRENAL MEDULLA; CHROMAFFIN GRANULES; CHROMAFFIN CELLS; ATP; ADENINE NUCLEOTIDES; BLOOD PLATELETS;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The level of diadenosine 5',5'''-P-1-P-4-tetraphosphate (diadenosine tetraphosphate or Ap(4)A), catecholamines, ATP and other nucleotides has been investigated in perchloric acid extracts of bovine adrenal medulla, chromaffin granules and cultured chromaffin cells. As a control, the amount of Ap(4)A and ATP has also been measured in human blood platelets. The following values (nmol/mg protein) were found in adrenal medulla: Ap(4)A, 0.019 +/- 0.004; ATP, 109 +/- 11; ADP, 23.8 +/- 5.8; AMP, 11.3 +/- 1.5; p(4)A, 0.18 +/- 0.08; catecholamines, 460 +/- 57. The level of Ap(4)A, catecholamines and ATP (nmol/mg protein) found in chromaffin granules and in chromaffin cells were, respectively: (0.15 +/- 0.07; 2175 +/- 99; 531 +/- 66) and (0.22 +/- 0.14; 1143 +/- 277; 222 +/- 53). In all the cases investigated, the ratio catecholamines/ATP and catecholamines/Ap(4)A were around 5 and in the order of 10(3), respectively. The amount of Ap(4)A found here, in bovine adrenal medulla, chromaffin granules and chromaffin cells, is two orders of magnitude lower than previously reported.

引用

页码：404 / 409

页数：6

共 28 条

[11] ADENOSINE 5'-TETRAPHOSPHATE AND ADENOSINE 5'-PENTAPHOSPHATE ARE SYNTHESIZED BY YEAST ACETYL-COENZYME-A SYNTHETASE [J].

GURANOWSKI, A ;

SILLERO, MAG ;

SILLERO, A .

JOURNAL OF BACTERIOLOGY, 1994, 176 (10) :2986-2990

[12]

GURANOWSKI A, 1992, AP4A OTHER DINUCLEOS, P81

[13]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[14] SEPARATION AND CULTURE OF LIVING ADRENALINE-CONTAINING AND NORADRENALINE-CONTAINING CELLS FROM BOVINE ADRENAL MEDULLAE [J].

MORO, MA ;

LOPEZ, MG ;

GANDIA, L ;

MICHELENA, P ;

GARCIA, AG .

ANALYTICAL BIOCHEMISTRY, 1990, 185 (02) :243-248

[15] CHARACTERIZATION OF 2 CHROMAFFIN CELL-POPULATIONS ISOLATED FROM BOVINE ADRENAL-MEDULLA [J].

MORO, MA ;

GARCIA, AG ;

LANGLEY, OK .

JOURNAL OF NEUROCHEMISTRY, 1991, 57 (02) :363-369

[16] SPECIFIC SYNTHESIS OF ADENOSINE (5')TETRAPHOSPHO(5')NUCLEOSIDE AND ADENOSINE(5')OLIGOPHOSPHO(5')ADENOSINE (N-GREATER-THAN-4) CATALYZED BY FIREFLY LUCIVERASE [J].

ORTIZ, B ;

SILLERO, A ;

SILLERO, MAG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (01) :263-270

[17] PRESENCE OF DIADENOSINE POLYPHOSPHATES - AP4A AND AP5A - IN RAT-BRAIN SYNAPTIC TERMINALS - CA2+ DEPENDENT RELEASE EVOKED BY 4-AMINOPYRIDINE AND VERATRIDINE [J].

PINTOR, J ;

DIAZREY, MA ;

TORRES, M ;

MIRASPORTUGAL, MT .

NEUROSCIENCE LETTERS, 1992, 136 (02) :141-144

[18] CHARACTERIZATION AND QUANTIFICATION OF DIADENOSINE HEXAPHOSPHATE IN CHROMAFFIN CELLS - GRANULAR STORAGE AND SECRETAGOGUE-INDUCED RELEASE [J].

PINTOR, J ;

ROTLLAN, P ;

TORRES, M ;

MIRASPORTUGAL, MT .

ANALYTICAL BIOCHEMISTRY, 1992, 200 (02) :296-300

[19] CARBACHOL INDUCED RELEASE OF DIADENOSINE POLYPHOSPHATES - AP4A AND AP5A - FROM PERFUSED BOVINE ADRENAL-MEDULLA AND ISOLATED CHROMAFFIN CELLS [J].

PINTOR, J ;

TORRES, M ;

MIRASPORTUGAL, MT .

LIFE SCIENCES, 1991, 48 (24) :2317-2324

[20] ZINC-DEPENDENT SYNTHESIS OF VARIOUS DINUCLEOSIDE 5',5'''-P-1,P-3-TRI-TETRAPHOSPHATE OR 5',5'''-P-1,P-4-TETRAPHOSPHATE BY ESCHERICHIA-COLI LYSYL-TRNA SYNTHETASE [J].

PLATEAU, P ;

BLANQUET, S .

BIOCHEMISTRY, 1982, 21 (21) :5273-5279

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