SELECTION AND CHARACTERIZATION OF INHIBITOR-RESISTANT MUTANTS OF PHYTOPHTHORA-SOJAE

被引：2
作者：
BHAT, RG
SCHMITTHENNER, AF
机构：
[1] Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster
来源：
EXPERIMENTAL MYCOLOGY | 1993年 / 17卷 / 02期
关键词：
PHYTOPHTHORA-MEGASPERMA F SP GLYCINEA; PHYTOPHTHORA-SOJAE; PHYSIOLOGICAL RACES; SOYBEAN; INDUCED MUTATION; INHIBITOR-RESISTANT MUTANTS; METALAXYL; P-FLUOROPHENYLALANINE;
D O I：
10.1006/emyc.1993.1010
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Bhat, R. G., and Schmitthenner, A. F. 1993. Selection and characterization of inhibitor-resistant mutants of Phytophthora sojae. Experimental Mycology 17, 109-121. Selectable markers, resistance to metalaxyl (MEX) and p -fluorophenylalanine (FPA), were induced in Phytophthora sojae races by treating zoospores with N -methyl-N′-nitro-N′-nitrosoguanidine. Calcium treatment enhanced the percentage encystment of zoospores. MEX-resistant (MEXr) and FPA-resistant (FPAr) mutants were selected on a lima bean agar medium containing MEX (10 μg/ml) and FPA (50 μg/ml), respectively. A number of single inhibitor-resistant mutants were obtained. There was variation in in vitro growth of mutants. Virulence of mutants was evaluated by hypocotyl inoculation of soybean cultivars with Rps 1 (Amsoy 71), Rps1-c (Williams 79), Rps1-k (Williams 82), or rps1 (Williams) alleles. It was observed that the 23% of the MEXr and 33% of the FPAr mutants had a different virulence pattern than the original cultures. © 1993 Academic Press. All rights reserved.
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页码：109 / 121
页数：13
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