ELECTRON-IMPACT AND CHEMICAL IONIZATION DETECTION OF NICOTINE AND COTININE BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY IN RAT PLASMA AND BRAIN

被引：27

作者：

DEUTSCH, J ^{[1
]}

HEGEDUS, L ^{[1
]}

GREIG, NH ^{[1
]}

RAPOPORT, SI ^{[1
]}

SONCRANT, TT ^{[1
]}

机构：

[1] NIA,NEUROSCI LAB,10-6C103,BETHESDA,MD 20892

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 579卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80366-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nicotine and its metabolite, cotinine, were measured in rat plasma and brain by gas chromatography mass spectrometry. Both agents were extracted from plasma and brain, separated on a capillary column, and quantified by single-ion monitoring. The major fragment ions of nicotine and cotinine at m/z 84 and m/z 98, respectively, were monitored by electron-impact ionization detection and the protonated molecular ions at m/z 163 and m/z 177, respectively, were monitored by chemical ionization detection. Both compounds were quantified using deuterium-labeled nicotine and cotinine, respectively, as internal standards.

引用

页码：93 / 98

页数：6

共 16 条

[1] SIMULTANEOUS DETERMINATION OF NICOTINE AND COTININE IN PLASMA USING CAPILLARY COLUMN GAS-CHROMATOGRAPHY WITH NITROGEN-SENSITIVE DETECTION [J].