FACTORS AFFECTING FORMATION OF SOMATIC EMBRYOS AND EMBRYOGENIC CALLUS FROM UNEMERGED INFLORESCENCES OF A GRAMINEOUS CROP PENNISETUM

被引:8
作者
TALWAR, M [1 ]
RASHID, A [1 ]
机构
[1] UNIV DELHI,DEPT BOT,DELHI 110007,INDIA
关键词
Embryogenic callus; Pennisetum; Somatic embryo; Unemerged inflorescence;
D O I
10.1093/oxfordjournals.aob.a087995
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Differentiation of somatic embryos was dependent on the concentration of auxin and the mineral medium. Low levels of auxin 2,4-D in N6 medium, a low ammonium nutrient, favoured the formation of somatic embryos, while on MS medium containing high ammonium compact tissues appeared. At higher levels of auxin, irrespective of nutrient medium, compact tissues were formed. The origin of compact tissue on N4 medium could be traced to somatic embryo-like structures. This tissue regenerated into somatic embryos on hormone-free N6 medium whereas on MS medium thalloid structures appeared. © 1990 Annals of Botany Company.
引用
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页码:17 / 21
页数:5
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