LEISHMANIA - GENUS IDENTIFICATION BASED ON A SPECIFIC SEQUENCE OF THE 18S RIBOSOMAL-RNA SEQUENCE

被引：85
作者：
ULIANA, SRB
AFFONSO, MHT
CAMARGO, EP
FLOETERWINTER, LM
机构：
[1] Departamento de Parasitologia, Institute de Ciências Biomédicas, Universidade de São Paulo, CEP 01051 São Paulo, SP
来源：
EXPERIMENTAL PARASITOLOGY | 1991年 / 72卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
LEISHMANIA; GENUS IDENTIFICATION; RIBOSOMAL DNA (RDNA); OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION (PCR); RIBOSOMAL RNA (RIBOSOMAL-RNA); KINETOPLAST DNA (KDNA); STANDARD SALINE CITRATE (SSC); DITIOTHREITOL (DTT); PHOSPHATE-BUFFERED SALINE (PBS);
D O I：
10.1016/0014-4894(91)90133-H
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The analysis of PvuII restriction patterns of Leishmania spp. and Trypanosoma spp. genomic DNA showed genus distinctive profiles. A specific PvuII site was detected in the 5′ domain of 18S ribosomal DNA of Leishmania. A 20-mer oligonucleotide encompassing this PvuII region was synthesized. This sequence, when utilized as probe, on short exposures of dot tests, detected 103 whole promastigotes of all Leishmania species analyzed but did not hybridize with T. cruzi or human nucleic acids. Two other oligonucleotides were synthesized to be used as primers for amplification through polymerase chain reaction of the 18S ribosomal DNA region containing the PvuII site. The probes described may be useful for the detection of Leishmania spp. under clinical and epidemiological trials. © 1991.
引用
收藏
页码：157 / 163
页数：7
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