THE ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM WHEAT, BARLEY AND RYE FOR ANALYSIS BY PULSE-FIELD GEL-ELECTROPHORESIS

被引：31
作者：
CHEUNG, WY
GALE, MD
机构：
[1] Cambridge Laboratory, Norwich, NR4 7UJ, Colney Lane
来源：
PLANT MOLECULAR BIOLOGY | 1990年 / 14卷 / 05期
关键词：
cereals; high molecular weight DNA; pulse-field gel electrophoresis;
D O I：
10.1007/BF00016525
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is presented for the preparation of large DNA molecules from protoplasts embedded in agarose blocks of three different cereals-hexaploid bread wheat (Triticum aestivum), barley (Hordeum vulgare) and rye (Secale cereale). Pulse-field gel electrophoresis (PFGE) analysis of these DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA molecules was greater than 6 Mb. DNA samples prepared by this method were shown to be useful for restriction analysis using both frequent and rare cutting enzymes. © 1990 Kluwer Academic Publishers.
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页码：881 / 888
页数：8
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