CLONING OF GENES FOR SPERMINE RESISTANCE IN SACCHAROMYCES-CEREVISIAE AND THEIR EFFECTS ON S-ADENOSYL-L-METHIONINE ACCUMULATION

Among the polyamines tested, spermine strongly inhibited the growth of Saccharomyces cerevisiae DKD-5D-H. Two kinds of DNA fragments that confer strong and weak resistances to spermine were cloned onto a vector plasmid, YEp13. The restriction map of the DNA fragment conferring strong resistance was the same as that of a DNA fragment responsible for ethionine resistance in the same yeast cells. (Shiomi et al., Appl. Microbiol. Biotechnol., 29, 302-304, 1988) The yeast cells with the DNA fragment conferring strong resistance to spermine were resistant to ethionine and accumulated S-adenosyl-l-methionine intracellularly. © 1989.