REGULATION OF CYTOSOLIC CALCIUM BY ANGIOTENSINS IN VASCULAR SMOOTH-MUSCLE

被引:78
|
作者
DOSTAL, DE [1 ]
MURAHASHI, T [1 ]
PEACH, MJ [1 ]
机构
[1] UNIV VIRGINIA,SCH MED,DEPT PHARMACOL,BOX 448,JORDAN HALL,CHARLOTTESVILLE,VA 22908
关键词
Angiotensins; Calcium; Fluorescent indicators; Inositol phosphates; Pertussis toxin; Vascular smooth muscle;
D O I
10.1161/01.HYP.15.6.815
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
The carboxy terminal homologue of angiotensin ? (Aug II), Ang-(3-8) or hexa peptide, was used as a model peptide to examine the types of receptor mechanisms involved in calcium mobilization in cultured vascular smooth muscle cells. Hexapepdde did not produce tachyphylaxis but did produce a sustained increase in intracellular calcium. Differences in the increase in intracellular calcium ([Ca2+]1) and the pattern of inositol phosphate production indicate that Ang-(3-8) and maxima] concentrations of Ang II mobilize calcium through different mechanisms. The calcium-mobilizing mechanisms that predominate appear to depend on the concentration of angiotensin. Concentrations of Ang II greater than I0-8M produce sharp calcium transients in which the [Ca2+]1 returns close to baseline within 1 minute after stimulation, but concentrations of Ang II equal to or less than 3x10-9 M result in a plateau increase in calcium. Pretreatment with Bordetella pertussis toxin does not abolish either the calcium transient induced by Ang II or the plateau phase induced by Ang-(3-8), indicating that the GTP-transducing protein that couples the receptor to phospholipase C or, possibly, a receptor-operated calcium channel is not Bordetella pertussis toxin sensitive. © 1990 American Heart Association, Inc.
引用
收藏
页码:815 / 822
页数:8
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