BIOLOGICAL PROPERTIES AND PARTIAL-PURIFICATION OF A GROWTH-FACTOR FROM PORCINE SPLEEN

被引：4
作者：
DITTRICH, W [1 ]
ROTHE, H [1 ]
JAROS, PP [1 ]
WILLIG, A [1 ]
机构：
[1] UNIV OLDENBURG,DEPT ZOOPHYSIOL,PB 2503,W-2900 OLDENBURG,GERMANY
来源：
EXPERIMENTAL CELL RESEARCH | 1990年 / 188卷 / 01期
关键词：
D O I：
10.1016/0014-4827(90)90295-L
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
3T3-Fibroblast growth is enhanced in a dose-dependent way by a factor isolated from porcine spleen after limited proteolysis. The factor is not dialyzable and is stable for 5 min at 100 °C and at pH 2 at room temperature. Trypsin or collagenase treatment does not affect its biological activity. The stimulation of cell growth is independent of serum concentration in the culture medium but is accelerated by insulin supplement. The biochemical properties of this factor indicate a novel growth promotor different from other growth factors. © 1990.
引用
收藏
页码：172 / 174
页数：3
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