SELECTIVE ELUTION AND PURIFICATION OF LIVING TRICHOMONAS-VAGINALIS USING GRAVITATIONAL FIELD-FLOW FRACTIONATION

被引：40
作者：
BERNARD, A
BORIES, C
LOISEAU, PM
CARDOT, PJP
机构：
[1] UNIV PARIS 11,CTR ETUD PHARMACEUT,CHIM ANALYT & ELECTROCHIM ORGAN LAB,F-92296 CHATENAY MALABRY,FRANCE
[2] UNIV PARIS 11,CTR ETUD PHARMACEUT,PARASITOL LAB,F-92296 CHATENAY MALABRY,FRANCE
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期
关键词：
D O I：
10.1016/0378-4347(94)00498-T
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gravitational held-how fractionation is one of the simplest separation methods for biological materials. Its potential in parasitology is demonstrated for Trichomonas vaginalis, a parasite responsible for one of the most widespread sexually transmitted diseases. It was observed that this unicellular parasite can be purified in a culture medium with a recovery of 85% for the living trophozoites. The parasite retention characteristics were different when motile living and non-motile dead cells were eluted, motile cells being less retained than the non-motile cells.
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收藏
页码：444 / 448
页数：5
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