ACTIVATION OF HUMAN NEUTROPHILS BY PHORBOL ESTER DECREASES THE CYTOPLASM COMPACTNESS AND THE LACTOFERRIN CONTENT OF THE GRANULOCYTES

被引:20
作者
ESAGUY, N
AGUAS, AP
VILANOVA, M
SILVA, MT
机构
[1] UNIV PORTO, ABEL SALAZAR INST BIOMED SCI, DEPT ANAT, P-4100 OPORTO, PORTUGAL
[2] UNIV PORTO, ABEL SALAZAR INST BIOMED SCI, DEPT IMMUNOL, P-4100 OPORTO, PORTUGAL
关键词
SECRETION; PROTEIN KINASE-C; IRON-BINDING PROTEINS; CYTOCHEMISTRY; IMMUNOGOLD; ELECTRON MICROSCOPY;
D O I
10.1002/jlb.50.5.444
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Human neutrophils isolated from the blood were incubated in vitro for 30 min with 25 ng/ml of phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, to study its effect on the fine structure of granulocytes and on the subcellular distribution of lactoferrin (Lf). Flow cytometry analysis of the human neutrophils showed that PMA induced a decrease in size and granularity of the cells. By electron microscopy, the PMA-treated cells showed numerous empty vesicles, smoothing of the cell surface, and a marked decrease in the compactness of the cytoplasmic ground substance. High-resolution immunogold ultracytochemistry showed that neutrophils lost most of their content in Lf after the PMA incubation. In thin sections, the cytoplasmic compartment of PMA-treated neutrophils contained an average of 1.03 +/- 0.3 Lf-positive vesicles per-mu-m2, whereas control granulocytes showed an average of 5.72 +/- 1.49 Lf vesicles per-mu-m2 of cytoplasm. Most of PMA-treated neutrophils kept some Lf-positive vesicles; a significant minority (10-15%) of the granulocytes showed cytological features of cell death. We conclude that the PMA-induced neutrophil activation is associated with microanatomical changes that include i) exocytosis of most, but not all, of the Lf-bearing vesicles; ii) rounding up of the cell outline; and iii) decrease in the compactness of the cytoplasmic ground substance.
引用
收藏
页码:444 / 452
页数:9
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