ISOLATION OF HOMOGENEOUS PHOSPHOLIPASE-A2 FROM HUMAN-PLATELETS

被引:65
作者
APITZCASTRO, RJ [1 ]
MAS, MA [1 ]
CRUZ, MR [1 ]
JAIN, MK [1 ]
机构
[1] UNIV DELAWARE,DEPT CHEM,NEWARK,DE 19711
基金
美国国家科学基金会;
关键词
D O I
10.1016/0006-291X(79)90583-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phospholipase A2 from human blood platelets has been purified to homogeneity by treatment of the platelet homogenate with H2SO4, followed by affinity chromatography of the extract. The platelet phospholipase is a soluble, heat-stable protein of MW 44000. The purified enzyme inhibits ADP-induced platelet aggregation. © 1979.
引用
收藏
页码:63 / 71
页数:9
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