INTERPRETATION OF NUCLEAR MAGNETIC-RESONANCE SPECTRA FOR LACTOBACILLUS-CASEI DIHYDROFOLATE-REDUCTASE BASED ON THE X-RAY STRUCTURE OF THE ENZYME-METHOTREXATE-NADPH COMPLEX

被引:25
作者
MATTHEWS, DA
机构
[1] Department of Chemistry, University of California, San Diego, La Jolla
关键词
D O I
10.1021/bi00575a035
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The three-dimensional molecular structure of Lactobacillus casei dihydrofolate reductase complexed with NADPH and methotrexate has been used to interpret published magnetic resonance spectra for this enzyme. Proton resonances from histidine residues and 19F resonances from fluorine-labeled fluorotyrosine and fluorotryptophan dihydrofolate reductase have been assigned in several cases to specific amino acids in the primary sequence. Furthermore, the 31P signals from the pyrophosphate moiety of bound NADPH have been assigned and the large upfield shift for l3C-labeled (at the carboxamide carbon) NADP+ upon binding to the reductase has been explained in terms of desolvation effects. © 1979, American Chemical Society. All rights reserved.
引用
收藏
页码:1602 / 1610
页数:9
相关论文
共 29 条
  • [21] INTERACTION OF C-13-ENRICHED FOLATE WITH DIHYDROFOLATE-REDUCTASE STUDIED BY CARBON MAGNETIC-RESONANCE SPECTROSCOPY
    PASTORE, EJ
    PLANTE, LT
    WRIGHT, JM
    KISLIUK, RL
    KAPLAN, NO
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 68 (02) : 471 - 475
  • [22] CONFORMATIONAL-CHANGES INDUCED IN DIHYDROFOLATE-REDUCTASE BY FOLATES, PYRIDINE-NUCLEOTIDE COENZYMES, AND METHOTREXATE
    PASTORE, EJ
    KISLIUK, RL
    PLANTE, LT
    WRIGHT, JM
    KAPLAN, NO
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (10) : 3849 - 3853
  • [23] POE M, 1974, CANCER BIOCHEM BIOPH, V1, P7
  • [24] NUCLEAR MAGNETIC-RESONANCE STUDIES OF BINDING OF SUBSTRATE ANALOGS AND COENZYME TO DIHYDROFOLATE-REDUCTASE FROM LACTOBACILLUS-CASEI
    ROBERTS, GCK
    FEENEY, J
    BURGEN, ASV
    YUFEROV, V
    DANN, JG
    BJUR, R
    [J]. BIOCHEMISTRY, 1974, 13 (26) : 5351 - 5357
  • [25] BINDING OF METHOTREXATE TO ESCHERICHIA-COLI DIHYDROFOLATE-REDUCTASE AS MEASURED BY VISIBLE AND ULTRAVIOLET RESONANCE RAMAN-SPECTROSCOPY
    SAPERSTEIN, DD
    REIN, AJ
    POE, M
    LEAHY, MF
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1978, 100 (13) : 4296 - 4300
  • [26] SARMA RH, 1973, CONFORMATION BIOL MO, P591
  • [27] STOTHERS JB, 1972, CARBON 13 NMR SPECTR, P494
  • [28] INTERACTION OF METHOTREXATE, FOLATES, AND PYRIDINE-NUCLEOTIDES WITH DIHYDROFOLATE-REDUCTASE - CALORIMETRIC AND SPECTROSCOPIC BINDING STUDIES
    SUBRAMANIAN, S
    KAUFMAN, BT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (07) : 3201 - 3205
  • [29] NUCLEAR MAGNETIC-RESONANCE STUDY OF NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE BINDING TO LACTOBACILLUS-CASEI DIHYDROFOLATE-REDUCTASE
    WAY, JL
    BIRDSALL, B
    FEENEY, J
    ROBERTS, GCK
    BURGEN, ASV
    [J]. BIOCHEMISTRY, 1975, 14 (15) : 3470 - 3475