ATP-DRIVEN POTASSIUM-TRANSPORT IN RIGHT-SIDE-OUT MEMBRANE-VESICLES VIA THE KDP SYSTEM OF ESCHERICHIA-COLI

被引：32
|
作者：
KOLLMANN, R [1 ]
ALTENDORF, K [1 ]
机构：
[1] UNIV OSNABRUCK, FACHBEREICH BIOL CHEM, ARBEITSGRP MIKROBIOL, BARBARASTR 11, W-4500 OSNABRUCK, GERMANY
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1143卷 / 01期
关键词：
KDP-ATPASE; POTASSIUM TRANSPORT; RIGHT-SIDE-OUT MEMBRANE VESICLE; PHOSPHOGLYCERATE TRANSPORT; ATP GENERATION; (ESCHERICHIA-COLI);
D O I：
10.1016/0005-2728(93)90216-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ATP-generating system described by Hugenholtz, J., Hong, J.-S. and Kaback, H.R. ((1981) Proc. Natl. Acad. Sci. USA 78, 3446-3449) has been used to synthesize ATP up to 1.8 mM in right-side-out membrane vesicles from Escherichia coli. This ATP level was sufficient to drive uptake of potassium ions via the Kdp-ATPase. In the kdp wild type strain about 110 nmoles K+/mg membrane protein were accumulated. This process was still partially sensitive to the well-known inhibitors of P-type ATPases, orthovanadate and bafilomycin B1.
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页码：62 / 66
页数：5
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