ATP-DRIVEN POTASSIUM-TRANSPORT IN RIGHT-SIDE-OUT MEMBRANE-VESICLES VIA THE KDP SYSTEM OF ESCHERICHIA-COLI

被引：32

作者：

KOLLMANN, R ^{[1
]}

ALTENDORF, K ^{[1
]}

机构：

[1] UNIV OSNABRUCK, FACHBEREICH BIOL CHEM, ARBEITSGRP MIKROBIOL, BARBARASTR 11, W-4500 OSNABRUCK, GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1143卷 / 01期

关键词：

KDP-ATPASE; POTASSIUM TRANSPORT; RIGHT-SIDE-OUT MEMBRANE VESICLE; PHOSPHOGLYCERATE TRANSPORT; ATP GENERATION; (ESCHERICHIA-COLI);

D O I：

10.1016/0005-2728(93)90216-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ATP-generating system described by Hugenholtz, J., Hong, J.-S. and Kaback, H.R. ((1981) Proc. Natl. Acad. Sci. USA 78, 3446-3449) has been used to synthesize ATP up to 1.8 mM in right-side-out membrane vesicles from Escherichia coli. This ATP level was sufficient to drive uptake of potassium ions via the Kdp-ATPase. In the kdp wild type strain about 110 nmoles K+/mg membrane protein were accumulated. This process was still partially sensitive to the well-known inhibitors of P-type ATPases, orthovanadate and bafilomycin B1.

引用

页码：62 / 66

页数：5

共 32 条

[1]

Bakker E.P., 1992, ALKALI CATION TRANSP

[2] EVIDENCE FOR MULTIPLE K+ EXPORT SYSTEMS IN ESCHERICHIA-COLI [J].