INDUCTION TEMPERATURE OF HUMAN HEAT-SHOCK FACTOR IS REPROGRAMMED IN A DROSOPHILA CELL ENVIRONMENT

被引：78

作者：

CLOS, J ^{[1
]}

RABINDRAN, S ^{[1
]}

WISNIEWSKI, J ^{[1
]}

WU, C ^{[1
]}

机构：

[1] NCI,BIOCHEM LAB,BLDG 37,RM 4C-09,BETHESDA,MD 20892

来源：

NATURE | 1993年 / 364卷 / 6434期

关键词：

D O I：

10.1038/364252a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

HEAT shock factor (HSF)1,2, the transcriptional activator of eukaryotic heat shock genes, is induced to bind DNA by a monomer to trimer transition involving leucine zipper interactions3,4. Although this mode of regulation is shared among many eukaryotic species, there is variation in the temperature at which HSF binding activity is induced. We investigated the basis of this variation by analysing the response of a human HSF expressed in Drosophila cells and Drosophila HSF expressed in human cells. We report here that the temperature that induces DNA binding and trimerization of human HSF in Drosophila was decreased by approximately 10-degrees-C to the induction temperature for the host cell, whereas Drosophila HSF expressed in human cells was constitutively active. The results indicate that the activity of HSF in vivo is not a simple function of the absolute environmental temperature.

引用

页码：252 / 255

页数：4

共 16 条

[1] ATTENUATION OF THE HEAT-SHOCK RESPONSE IN HELA-CELLS IS MEDIATED BY THE RELEASE OF BOUND HEAT-SHOCK TRANSCRIPTION FACTOR AND IS MODULATED BY CHANGES IN GROWTH AND IN HEAT-SHOCK TEMPERATURES [J].