DETERMINATION OF PROTEIN-BOUND DODECYL-SULFATE BY GAS-CHROMATOGRAPHY

被引：7

作者：

SARASTE, M ^{[1
]}

KORHONEN, TK ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT GEN MICROBIOL,SF-00290 HELSINKI 29,FINLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90682-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sodium dodecyl sulfate has been determined by a gas chromatographic method. The sulfate group is hydrolyzed, and the liberated dodecanol is determined by gas chromatography using an SE-30 column. Dodecanol is clearly resolved from the common fatty acid methyl esters. The presence of proteins or phospholipids in the sample seems to have no effect on the determination. Using a conventional gas chromatograph, 0.5 to 1.0 μg of dodecyl sulfate in the sample can be detected. © 1979.

引用

页码：444 / 446

页数：3

共 11 条

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