MORE SENSITIVE ASSAY DISCRIMINATING GALACTOSAMINE AND GLUCOSAMINE IN MIXTURES

被引：199

作者：

WAGNER, WD ^{[1
]}

机构：

[1] WAKE FOREST UNIV,BOWMAN GRAY SCH MED,ARTERIOSCLEROSIS RES CTR,WINSTON SALEM,NC 27103

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90379-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of a colorimetric assay (1) discriminating galactosamine and glucosamine in mixtures is presented. The procedure employs acetylation of the amino sugars at 25°C instead of at 0-1°C, resulting in five times the color intensity for galactosamine with no interference due to glucosamine. The assay of HCl hydrolysates of glycosaminoglycans and glycoproteins is possible in solutions that are less than 0.1 n. © 1979.

引用

页码：394 / 396

页数：3

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