AGE-DEPENDENT CHANGE IN MYOCARDIAL CARDIAC GLYCOSIDE RECEPTOR (NA,K-PUMP) CONCENTRATION IN CHILDREN

被引:19
作者
KJELDSEN, K [1 ]
GRON, P [1 ]
机构
[1] AARHUS UNIV,INST FORENS MED,DK-8000 AARHUS C,DENMARK
关键词
!sup]3[!/sup]H-ouabain-binding-site concentration; Cardiac glycoside receptors; Development; Myocardium; Na; K-ATPase; K-pumps;
D O I
10.1097/00005344-199002000-00022
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Myocardial Na, K-ATPase concentration was quantified in 18 0-8-year-old human subjects by vanadate-facilitated 3H-ouabain binding to intact samples of the left ventricle of the heart obtained at autopsy. Within the first 6 months of life, the Na, K-ATPase concentration showed a rapid decrease. The mean value in the age range from birth to 6 months was 1.6 times the mean value obtained in the age range 6 months to 8 years. The mean values ± SEM were 1,076 ± 57 (n = 10) and 671 ± 28 (n = 8) (p < 0.001) pmol/g wet weight, respectively. The highest value [1,433 ± 56 pmoUg wet weight (n = 5)] was found in a 3-month-old child and the lowest value [545 ± 22 pmol/g wet weight (n = 5)] in an 8-year-old. Evaluation of 3H-ouabain-binding kinetics showed no agedependent variations. The total amount of Na, K-ATPase found in the heart was ±30 and 80 nmol within the first 3 years of life and at 8 years, respectively. The age-dependent change in myocardial Na, K-ATPase concentration can be ascribed to variation in the ratio between the amount of Na, K-ATPase and muscle mass during development. Since myocardial Na, K-ATPase is the receptor for cardiac glycosides, the present results may in part explain the clinical observation that cardiac glycoside sensitivity and toxicity change in young age. © 1990 Raven Press, Ltd., New York.
引用
收藏
页码:332 / 337
页数:6
相关论文
共 27 条
[1]  
CHARLEMAGNE D, 1987, J BIOL CHEM, V262, P8941
[2]  
CHARLEMAGNE D, 1986, J BIOL CHEM, V261, P185
[3]   THE EFFECT OF ISCHEMIA AND REPERFUSION ON SARCOLEMMAL FUNCTION IN PERFUSED CANINE HEARTS [J].
CHEMNITIUS, JM ;
SASAKI, Y ;
BURGER, W ;
BING, RJ .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1985, 17 (12) :1139-1150
[4]   SERUM DIGITOXIN CONCENTRATIONS IN INFANTS AND CHILDREN [J].
GIARDINA, ACV ;
EHLERS, KH ;
MORRISON, JB ;
ENGLE, MA .
CIRCULATION, 1975, 51 (04) :713-717
[5]   AGE-RELATED-CHANGES IN OUABAIN PHARMACOLOGY - OUABAIN EXHIBITS A DIFFERENT VOLUME OF DISTRIBUTION IN ADULT AND YOUNG DOGS [J].
GLANTZ, SA ;
KERNOFF, R ;
GOLDMAN, RH .
CIRCULATION RESEARCH, 1976, 39 (03) :407-414
[6]   STUDY ON INFLUENCE OF CONCENTRATION OF MG2+, PI, K+, NA+, AND TRIS ON (MG2++PI)-SUPPORTED G-STROPHANTHIN BINDING TO (NA++K+)-ACTIVATED ATPASE FROM OX BRAIN [J].
HANSEN, O ;
SKOU, JC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 311 (01) :51-66
[8]  
HANSEN O, 1988, AM J PHYSIOL, V254, P7
[9]  
JONES LR, 1980, J BIOL CHEM, V255, P9971
[10]  
JONES NGB, 1984, ETHOL SOCIOBIOL, V5, P1