STUDIES ON INDIRECT THERMOOPTICAL DETECTION FOR CAPILLARY ELECTROPHORESIS

Indirect thermo-optical detection for capillary electrophoresis was presented first by combining indirect detection with thermo-optical method. In this detection, methylene blue solution was used as background electrolyte solution, because it has large molar absorption coefficient at 633 nm. This wavelength matches well with that of He-Ne laser(632.8 nm). The detection was applied to detect amino acids in capillary zone electrophoresis separation. The detection limit for lysine was 5X10(-6) mol/L (S/N=2).