EVALUATION OF BACILLUS-SUBTILIS STRAIN IS53 FOR THE PRODUCTION OF BACILLUS-ANTHRACIS PROTECTIVE ANTIGEN

被引：9

作者：

BAILLIE, LWJ

JOHNSON, M

MANCHEE, RJ

机构：

[1] Defence Microbiology Division, The Chemical and Biological Defence Establishment, Salisbury, Wiltshire, Porton Down

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00949.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An asporogenous strain of Bacillus subtilis, IS53, transformed with plasmid pPA102, produces the protective antigen (PA) component of the tripartite toxin of B. anthracis. Addition of yeast extract was required to support growth and PA production in all the media examined. Protective antigen expression was down-regulated during exponential growth and extracellular proteases caused marked degradation of the mature protein.

引用

页码：225 / 227

页数：3

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