INVIVO STRIATAL DOPAMINE RELEASE BY M1 MUSCARINIC RECEPTORS IS INDUCED BY ACTIVATION OF PROTEIN KINASE-C

被引：14

作者：

XU, M

YAMAMOTO, T

KATO, T

机构：

[1] YOKOHAMA CITY UNIV,GRAD SCH INTEGRATED SCI,MOLEC RECOGNIT LAB,22-2 SETO,KANAZAWA KU,YOKOHAMA 236,JAPAN

[2] TOKYO INST TECHNOL,GRAD SCH NAGATSUTA,DEPT LIFE CHEM,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 54卷 / 06期

关键词：

Forskolin; In vivo striatal dopamine release; Islet‐activating protein; M1 muscarinic receptor; Polymyxin;

D O I：

10.1111/j.1471-4159.1990.tb04891.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: Islet‐activating protein was unilaterally microinjected into rat stratum, and a dialysis cannula was implanted into the same area under anesthesia. After 2 days, various agents were perfused continuously into the striatum through the dialysis membrane, under freely moving conditions. Isletactivating protein (2 μg/2 μ1) treatment alone did not change in vivo striatal dopamine (DA) release and metabolism, but completely abolished the increase of striatal DA release evoked in vivo by the M1‐selective agonist McN‐A‐343 (10−7M). Forskolin (10−5M), an adenylate cyclase activator, increased DA release and showed an additive effect on the DA release evoked by McN‐A‐343. Polymyxin B, a rather selective inhibitor of protein kinase C, decreased DA release and completely blocked the effect of McN‐A‐343. These results suggest that in vivo striatal DA release elicited by M1 muscarinic receptors is coupled with interaction with a G protein and is induced by activation of protein kinase C. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1917 / 1919

页数：3

共 8 条

[1] PHOSPHORYLATION OF SYNTHETIC RANDOM POLYPEPTIDES BY PROTEIN KINASE-P AND OTHER PROTEIN-SERINE (THREONINE) KINASES AND STIMULATION OR INHIBITION OF KINASE-ACTIVITIES BY MICROBIAL TOXINS [J].