EXPRESSION OF HUMAN POLY(ADP-RIBOSE) POLYMERASE IN SACCHAROMYCES-CEREVISIAE

被引：15

作者：

COLLINGE, MA ^{[1
]}

ALTHAUS, FR ^{[1
]}

机构：

[1] UNIV ZURICH, TIERSPITAL, INST BIOCHEM & PHARMACOL, CH-8057 ZURICH, SWITZERLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1994年 / 245卷 / 06期

关键词：

YEAST; SACCHAROMYCES CEREVISIAE; POLY(ADP-RIBOSE) POLYMERASE; DNA REPAIR;

D O I：

10.1007/BF00297275

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The coding sequence for human poly(ADP-ribose) polymerase was expressed inducibly in Saccharomyces cerevisiae from a low-copy-number plasmid vector. Cell free extracts of induced cells had poly(ADP-ribose) polymerase activity when assayed under standard conditions; activity could not be detected in noninduced cell extracts. Induced cells formed poly(ADP-ribose) in vivo, and levels of these polymers increased when cells were treated with the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The cytotoxicity of this agent was increased in induced cells, and in vivo labelling with [H-3] adenine further decreased their viability. Increased levels of poly(ADP-ribose) found in cells treated with the alkylating agent were not accompanied by lowering of the NAD concentration.

引用

页码：686 / 693

页数：8

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