DETERMINATION OF MIRFENTANIL HYDROCHLORIDE (A-3508.HCL) IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：5

作者：

BUGGE, CJL ^{[1
]}

TUCKER, MD ^{[1
]}

GARCIA, DB ^{[1
]}

KVALO, LT ^{[1
]}

WILHELM, JA ^{[1
]}

机构：

[1] ANAQUEST INC,MURRAY HILL,NJ 07974

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1993年 / 11卷 / 09期

关键词：

MIRFENTANIL HYDROCHLORIDE; LC-MS; PHARMACOKINETICS; PLASMA; ASSAY VALIDATION;

D O I：

10.1016/0731-7085(93)80073-A

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the quantitation of mirfentanil hydrochloride (A-3508.HCl) in human plasma is presented for the first time, using LC-MS with single ion monitoring. The drug is extracted with a C-18 solid-phase cartridge and the extract is analysed using a 3 cm C-18 column connected to the ion source of a mass spectrometer via a thermospray interface. The intense ion produced by the protonated molecular ion at m/z 377 is detected by the mass spectrometer in positive-ion mode. The range of quantitation is 0.4-100 ng ml-1 from a 0.5 ml plasma sample. Results of assay validation are given. The method was used to analyse samples from a human pharmacokinetic study following intravenous administration of mirfentanil hydrochloride.

引用

页码：809 / 815

页数：7

共 14 条

[1] EVOLUTION OF THE 4-ANILIDOPIPERIDINE CLASS OF OPIOID ANALGESICS [J].