IDENTIFICATION OF RESIDUES INVOLVED IN THE BINDING OF METHIONINE BY ESCHERICHIA-COLI METHIONYL-TRANSFER-RNA SYNTHETASE

被引：27

作者：

FOURMY, D ^{[1
]}

MECHULAM, Y ^{[1
]}

BRUNIE, S ^{[1
]}

BLANQUET, S ^{[1
]}

FAYAT, G ^{[1
]}

机构：

[1] ECOLE POLYTECH,CNRS,BIOCHIM LAB,URA 240,F-91128 PALAISEAU,FRANCE

来源：

FEBS LETTERS | 1991年 / 292卷 / 1-2期

关键词：

METHIONYL-TRANSFER-RNA SYNTHETASE; METHIONINE; SPECTROPHOTOFLUORIMETRY;

D O I：

10.1016/0014-5793(91)80879-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparison of the amino-acid sequences of several methionyl-tRNA synthetases indicates the occurrence of a few conserved motifs, having a possible functional significance. The role of one of these motifs, centered at position 300 in the E. coli enzyme sequence, was assayed by the use of site-directed mutagenesis. Substitution of the His301 or Trp305 residues by Ala resulted in a large decrease in methionine affinity, whereas the change of Val298 into Ala had only a moderate effect. The catalytic rate of the enzyme was unimpaired by these substitutions. It is concluded that the above conserved amino-acid region is located at or close to the amino-acid binding pocket of methionyl-tRNA synthetase.

引用

页码：259 / 263

页数：5

共 21 条

[1] MECHANISM OF ACTION OF METHIONYL-TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI - MECHANISM OF AMINO-ACID ACTIVATION REACTION CATALYZED BY NATIVE AND TRYPSIN MODIFIED ENZYMES [J].

BLANQUET, S ;

FAYAT, G ;

WALLER, JP .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 44 (02) :343-351

[2] MECHANISM OF REACTION OF METHIONYL TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI - INTERACTION OF ENZYME WITH LIGANDS OF AMINO-ACID-ACTIVATION REACTION [J].

BLANQUET, S ;

WALLER, JP ;

FAYAT, G ;

IWATSUBO, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 24 (03) :461-&

[3] MECHANISM OF ACTION OF METHIONYL-TRANSFER RNA-SYNTHETASE FROM ESCHERICHIA-COLI .1. FLUORESCENCE STUDIES ON TRANSFER RNA MET BINDING AS A FUNCTION OF LIGANDS, IONS AND PH [J].

BLANQUET, S ;

IWATSUBO, M ;

WALLER, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 36 (01) :213-226

[4] AMINO-ACID ACTIVATION REACTION CATALYZED BY METHIONYL-TRANSFER RNA-SYNTHETASE - EVIDENCE FOR SYNERGISTIC COUPLING BETWEEN SITES FOR METHIONINE ADENOSINE AND PYROPHOSPHATE [J].

BLANQUET, S ;

FAYAT, G ;

WALLER, JP .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 94 (01) :1-15

[5] CRYSTALLOGRAPHIC STUDY AT 2.5A RESOLUTION OF THE INTERACTION OF METHIONYL-TRANSFER-RNA SYNTHETASE FROM ESCHERICHIA-COLI WITH ATP [J].

BRUNIE, S ;

ZELWER, C ;

RISLER, JL .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 216 (02) :411-424

[6]

CHATTON B, 1987, J BIOL CHEM, V262, P15094

[7] MOLECULAR-CLONING AND PRIMARY STRUCTURE OF THE ESCHERICHIA-COLI METHIONYL-TRANSFER RNA-SYNTHETASE GENE [J].

DARDEL, F ;

FAYAT, G ;

BLANQUET, S .

JOURNAL OF BACTERIOLOGY, 1984, 160 (03) :1115-1122

[8] PARTITION OF TRANSFER-RNA SYNTHETASES INTO 2 CLASSES BASED ON MUTUALLY EXCLUSIVE SETS OF SEQUENCE MOTIFS [J].

ERIANI, G ;

DELARUE, M ;

POCH, O ;

GANGLOFF, J ;

MORAS, D .

NATURE, 1990, 347 (6289) :203-206

[9] COUPLINGS BETWEEN SITES FOR METHIONINE AND ADENOSINE 5'-TRIPHOSPHATE IN AMINO-ACID ACTIVATION REACTION CATALYZED BY TRYPSIN-MODIFIED METHIONYL-TRANSFER RNA-SYNTHETASE FROM ESCHERICHIA-COLI [J].

FAYAT, G ;

FROMANT, M ;

BLANQUET, S .

BIOCHEMISTRY, 1977, 16 (11) :2570-2579

[10] DISSECTION OF THE STRUCTURE AND ACTIVITY OF THE TYROSYL-TRANSFER RNA-SYNTHETASE BY SITE-DIRECTED MUTAGENESIS [J].

FERSHT, AR .

BIOCHEMISTRY, 1987, 26 (25) :8031-8037

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