ISOLATED OLIGOPURINE TRACTS DO NOT SIGNIFICANTLY AFFECT THE BINDING OF DNA TO NUCLEOSOMES

被引：8

作者：

GETTS, RC ^{[1
]}

BEHE, MJ ^{[1
]}

机构：

[1] LEHIGH UNIV,DEPT CHEM,BETHLEHEM,PA 18015

来源：

BIOCHEMISTRY | 1992年 / 31卷 / 23期

关键词：

D O I：

10.1021/bi00138a020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleosomal-length DNA was constructed to contain one of two 10 bp oligopurine.oligopyrimidine sequences, either d(A10.T10) or d(G10.C10). The 146 base pair (bp) sequences were then each tandemly cloned. This allowed for the production of circularly-permuted sequence variants in which the oligopurine tract was located at eight different positions. The permuted sequences were then assayed for their ability to reconstitute into nucleosomes by competitive reconstitution. The results of the assay indicate that the free energy of nucleosome formation differs only by several tenths of a kilocalorie per mole for an oligopurine tract at any position along the DNA, including the central dyad region.

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页码：5380 / 5385

页数：6

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