Modeling analysis of GST (glutathione-S-transferases) from Wuchereria bancrofti and Brugia malayi

被引：21

作者：

Bhargavi, Rayavarapu ^{[1
]}

Vishwakarma, Siddharth ^{[2
]}

Murty, Upadhyayula Suryanarayana ^{[1
]}

机构：

[1] Indian Inst Chem Technol, Biol Div, Bioinformat Grp, Hyderabad, India

[2] RDVV, Dept PG Studies & Res Biol sci, Jabalpur, India

来源：

BIOINFORMATION | 2005年 / 1卷 / 01期

关键词：

Filarial parasites; GST (glutathione-S-transferases) homology modeling; structural deviations; active site residues;

D O I：

10.6026/97320630001025

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

GST (glutathione S-transferases) are a family of detoxification enzymes that catalyze the conjugation of reduced GSH (glutathione) to xenobiotic (endogenous electrophilic) compounds. GST from Wb (Wuchereria bancrofti) and Bm (Brugia malayi) are significantly different from human GST in sequence and structure. Thus, Wb-GST and Bm-GST are potential chemotherapeutic targets for antifilarial treatment. Comparison of modeled Wb and Bm GST with human GST show structural difference between them. Analysis of the active site residues for the binding of electrophilic co-substrates provides insight towards the design of parasite specific GST inhibitors.

引用

页码：25 / 27

页数：3

共 20 条

[1] β-carbonyl substituted glutathione conjugates as inhibitors of O-volvulus GST2 [J].

Brophy, PM ;

Campbell, AM ;

van Eldik, AJ ;

Teesdale-Spittle, PH ;

Liebau, E ;

Wang, MF .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2000, 10 (09) :979-981

[2] Induction of specific cell-mediated immunity in mice by oral immunization with Salmonella expressing Onchocerca volvulus glutathione S-transferase [J].

Catmull, J ;

Wilson, ME ;

Kirchhoff, LV ;

Metwali, A ;

Donelson, JE .

VACCINE, 1999, 17 (01) :31-39

[3] REFINED CRYSTAL-STRUCTURE OF PORCINE CLASS-PI GLUTATHIONE-S-TRANSFERASE (PGST P1-1) AT 2-CENTER-DOT-1 A-ANGSTROM RESOLUTION [J].

DIRR, H ;

REINEMER, P ;

HUBER, R .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 243 (01) :72-92

[4]

Gubler D.J., 1998, EMERG INFECT DIS, V1, P1

[5] Immunomodulatory properties of a phosphorylcholine-containing secreted filarial glycoprotein [J].

Harnett, W ;

Deehan, MR ;

Houston, KM ;

Harnett, MM .

PARASITE IMMUNOLOGY, 1999, 21 (12) :601-608

[6] DISCUSSION OF SOLUTION FOR BEST ROTATION TO RELATE 2 SETS OF VECTORS [J].

KABSCH, W .

ACTA CRYSTALLOGRAPHICA SECTION A, 1978, 34 (SEP) :827-828

[7]

Kelly K., 1999, J CHEM COMP GROUP

[8] Thioredoxin from Brugia malayi:: Defining a 16-kilodalton class of thioredoxins from nematodes [J].

Kunchithapautham, K ;

Padmavathi, B ;

Narayanan, RB ;

Kaliraj, P ;

Scott, AL .

INFECTION AND IMMUNITY, 2003, 71 (07) :4119-4126

[9] ASSESSMENT OF PROTEIN MODELS WITH 3-DIMENSIONAL PROFILES [J].

LUTHY, R ;

BOWIE, JU ;

EISENBERG, D .

NATURE, 1992, 356 (6364) :83-85

[10] Current status and future prospects of the Global Lymphatic Filariasis Programme [J].

Molyneux, DH ;

Taylor, MJ .

CURRENT OPINION IN INFECTIOUS DISEASES, 2001, 14 (02) :155-159

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